Journal: Journal of Cellular and Molecular Medicine

Article Title: CircRHOT1 mediated cell proliferation, apoptosis and invasion of pancreatic cancer cells by sponging miR‐125a‐3p

doi: 10.1111/jcmm.15572

Figure Lengend Snippet: CircRHOT1 is overexpressed and affects the biological function of pancreatic cancer cells. A, Relative expression of circRHOT1 in PDAC cells and human pancreatic ductal cell line cells was measured by RT‐qPCR. B, Relative expression levels of circRHOT1 after transfection of PANC‐1 cells were measured by RT‐qPCR. C, The viability of PANC‐1 cells after transfection was detected by Cell Counting Kit‐8. D, 5‐Ethynyl‐20‐deoxyuridine assays were used to detect cell proliferation after transfection. E, Colony formation assays were used to detect clonogenic ability of PANC‐1 cells after transfection. F, Transwell assays were used to detect cell invasion capacities in PANC‐1 cells after transfection. G, Flow cytometric assays were used to detect apoptosis of PANC‐1 cells after transfection. H, Flow cytometric assays were used to observe the cell cycle after transfection.* P < .05, ** P < .01

Article Snippet: The human pancreatic cancer cell lines (PANC‐1, SW1990, COLO357 and CF‐PAC1) and the human pancreatic ductal cell line (HPDE) were purchased from GeneChem (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Cell Counting

Journal: Journal of Cellular and Molecular Medicine

Article Title: CircRHOT1 mediated cell proliferation, apoptosis and invasion of pancreatic cancer cells by sponging miR‐125a‐3p

doi: 10.1111/jcmm.15572

Figure Lengend Snippet: MiR‐125a‐3p is crucial for regulating the biological function of PANC‐1 cells. A, Putative complementary sites within circRHOT1 and miR‐125a‐3p were predicted by TargetScan. B, Relative expression of miR‐125a‐3pin human pancreatic ductal cell line (HPDE) and PANC‐1 cells was measured by RT‐qPCR. C, Relative expression levels of miR‐125a‐3p in PANC‐1 cells after transfection were measured by RT‐qPCR. D, Cell Counting Kit‐8 assays were used to detect the viability of PANC‐1 cells after transfection. E, 5‐Ethynyl‐20‐deoxyuridine assays were used to detect cell proliferation after transfection. F, Colony formation assays were used to detect clonogenic ability in PANC‐1 cells after transfection G, Transwell assays were used to detect cell invasion capacities in PANC‐1 cells after transfection. H and I, Flow cytometric assays were used to detect apoptosis and to assess the cell cycle after transfection. * P < .05, ** P < .01

Article Snippet: The human pancreatic cancer cell lines (PANC‐1, SW1990, COLO357 and CF‐PAC1) and the human pancreatic ductal cell line (HPDE) were purchased from GeneChem (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Cell Counting

Journal: Journal of Cellular and Molecular Medicine

Article Title: CircRHOT1 mediated cell proliferation, apoptosis and invasion of pancreatic cancer cells by sponging miR‐125a‐3p

doi: 10.1111/jcmm.15572

Figure Lengend Snippet: CircRHOT1 regulates the expression of E2F3 by targeting miR‐125a‐3p in PANC‐1 cells. A, RT‐qPCR was used to measure the relative expression of miR‐125a‐3pin PANC‐1 cells after transfection. B, RT‐qPCR was used to measure the expression level of circRHOT1 in PANC‐1 cells after transfection. C, Dual‐luciferase reporter assays were used to test the putative complementary sites within circRHOT1 with miR‐125a‐3p. D, Predicted binding sites of miR‐125a‐3p in the E2F3 3ʹUTRby TargetScan. E, Dual‐luciferase reporter assays were used to test the putative complementary sites within miR‐125a‐3p and the E2F3 3ʹUTR. F and G, Relative expression of E2F3mRNA and protein in human pancreatic ductal cell line (HPDE) and PANC‐1 cells was measured by RT‐qPCR. H and I, RT‐qPCR and Western blotting were used to measure the expression level of E2F3in PANC‐1 cells after circRHOT1 knockdown. J and K, Relative expression of E2F3mRNA and protein measured by RT‐qPCR and Western blot after transfection. L and M, Relative E2F3mRNA and protein expression was measured by RT‐qPCR and Western blot after transfection. * P < .05, ** P < .01

Article Snippet: The human pancreatic cancer cell lines (PANC‐1, SW1990, COLO357 and CF‐PAC1) and the human pancreatic ductal cell line (HPDE) were purchased from GeneChem (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Luciferase, Binding Assay, Western Blot, Knockdown